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WebAdd 50 µL of 10× MES buffer (to 25 mM final concentration) and water to bring reaction up to 1.0 mL final volume. Add protein stock solution (Optimizing the amount of EDAC and protein for details) Resuspend the medium and incubate for approximately 15 min by end-over-end mixing or a benchtop device. Activation and coupling WebSep 2, 2024 · In order to effectively initiate a reaction, collisions must be sufficiently energetic (or have sufficient kinetic energy) to bring about this bond disruption. A … labview real-time 2018
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