WebAlthough CCCP is the most widely used experimental inducer of mitophagy, other compounds such as nicotinamide, fisetin (FIS) and 1,10-phenanthroline (PHEN) have been also described as mitophagy inducers. Citation 2,25,47 We thus conducted mitophagy assays in SH-SY5Y cells treated with these inducers for 6 h and 24 h (Fig. S3). WebApr 13, 2024 · 从对照组或10μM CCCP处理组分离线粒体，与从稳定表达GFP-tagged KIF5B和mCherry-tagged DYNLL1的细胞系中分离的S-100 胞液片段共孵育，洗脱和分析线粒体募集的外源标记蛋白。结果表明，CCCP处理组线粒体动力蛋白募集能力显著降低，KIF5B募集能力显著增强。

CCCP-induced mitochondrial dysfunction

WebMay 6, 2015 · CCCP is the most commonly used inducer of mitophagy in mammalian cells and is thought to exert its effects by reducing the mitochondrial membrane potential. 10 … WebMar 3, 2012 · These results suggest that Parkin-mediated mitophagy in neurons is a much slower process than in other cell types reported. Treating HeLa cells with 10µM CCCP or SH-SY5Y cells with 20µM CCCP can induce Parkin association with mitochondria in ~80% of cells within 1–2 hr [9–11, 17]. sivakarthikeyan songs download

New method to assess mitophagy flux by flow cytometry

WebSep 12, 2014 · AMBRA1–ActA is a powerful inducer of mitophagy Hitherto, in mammalian cells, overexpression of PARKIN in combination … WebMdivi-1 inhibited mitochondrial fission [41] and CCCP mediated mitochondrial fission and mitophagy [42]. Using these two controls in our study, we were able to screen PD153035, cetuximab, and ... WebDec 16, 2011 · CCCP induces mitophagy in an AMPK-independent manner. (A) YFP-Parkin and RFP-LC3 were transfected for 24 h in WT or AMPKα1/α2 DKO MEFs. Then, the cells were incubated with DMSO or CCCP (20 μM) for 8 h, and subjected to immunocytochemistry as described in Section 2. YFP-Parkin, green; RFP-LC3, red; … sivakarthikeyan programs video download