Phospho explorer antibody array

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August undefined, 2024

WebMay 6, 2014 · Several phosphokinase antibody arrays have been developed , including Phospho Explorer Array (Full Moon BioSystems), Signal Transduction AntibodyArray (Hypromatrix), Kinex Antibody Microarray (Kinexus Bioinformatics), Human Phospho-Kinase Antibody Array (R&D system), and Panorama Antibody Array XP725 (Sigma-Aldrich). Web46 rows · The Proteome Profiler Human Phospho-Kinase Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Phosphorylation of captured proteins is detected with biotinylated phospho-specific detection antibodies …

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WebApr 29, 2024 · Phosphoprotein profiling by the Phospho Explorer Antibody Array PEX100, which was designed and manufactured by Full Moon Biosystems, Inc. (Sunnyvale, CA), contains 1318 antibodies. Each of the antibodies has two replicates that are printed on coated glass microscope slide, along with multiple positive and negative controls. WebFeb 28, 2024 · Building antibody arrays using capture reagents targeting proteins in the signalling cascade of interest has enabled researchers to investigate changes in protein profiles and modifications in signalling pathways in … earl rush salsa

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WebOct 27, 2024 · Phospho Explorer antibody array experiments and analyses were performed as a custom service by E-Biogen (Ebiogen Inc., Seoul, Republic of Korea). Overall design HUVECs were serum-starved for 8 hours and then incubated with an anti-TGFβ1 antibody for 1 hour, after which HUVECs were stimulated with 1μg/ml DG-LRG1 for 30 minutes. WebWhat Array format do you want? All Arrays. 96-well standard 96-well Fast. 384-well. OpenArray. What type of primers are you looking for? PCR/Sanger Sequencing Primers) Enter target information. Enter / Upload Multiple Targets. Enter Single Sequence. Select a single species to search by sequence. What chromosome position are you interested in? ... WebApoptosis Phospho Antibody Array This array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study serine/threonine and tyrosine phosphorylation at specific sites. earl rutherford obituary

Cell Signalling - how is the Phospho Explorer antibody array used ...

Proteome Profiler Antibody Arrays

WebPhospho Explorer Array is a glass-based Array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples. It is a high-throughput ELISA based antibody microarray for qualitative/semi-quantitative protein … WebOne of the best selling antibody arrays with 269 key phospho-specific and site-specific antibodies from multiple cancer signaling pathways. Cytokine Profiling Array Featuring 310 cytokine related antibodies. Each antibody is printed with six replicates. Ideal for cytokine profiling and inflammation studies. Phospho Explorer Array css make text glowWebJun 4, 2024 · Phosphoprotein array analysis was performed using the Phospho Explorer Antibody Array (cat. no. PEX100; Full Moon BioSystems, Inc.). Signaling protein analysis was performed using the Signaling Explorer Antibody Array (cat. no. SET100; Full Moon BioSystems, Inc.) and the Explorer Antibody Array (cat. no. ASB600; Full Moon … css make text fill width

"WebSep 1, 2024 · Total RNA was extracted by chloroform and isopropanol precipitation. cDNA was obtained using the SuperScript III First-Strand Synthesis System (Invitrogen, 18080-051). qPCR analyses were performed with primers listed below using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725270) in at least duplicate in three … " - Phospho explorer antibody array

Phospho explorer antibody array

WebAug 3, 2024 · A phospho-protein array analysis revealed that PF-3758309 down-regulates the NF-κB signaling pathway, which provides the most likely mechanism by which PF-3758309 inhibits latency reversal. WebMar 18, 2024 · The Phospho Explorer Antibody Array kit used in this study was obtained from Full Moon BioSystems (catalog PEX100; Sunnyvale, CA, USA) and covered 1318 well-characterized antibodies, the phosphorylated and non-phosphorylated state of these proteins will be detected at the same time (Pulito et al. 2016 ).

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Webarray antibody pairs are optimised to provide the most accurate and reliable data. Human Cytokine Array Panel A (Cat# ARY005B) PBMCs untreated (top) or treated with ... p27/Kip1, PON2, Phospho-p53 (S15), Phospho-p53 (S46), Phospho-p53 (S392), Phospho-Rad17 (S635), SMAC/Diablo, Survivin, TNF RI/TNFRSF1A, TRAIL R1/DR4, TRAIL R2/DR5, XIAP ... WebFeb 12, 2024 · Phospho-protein array Screening of 1318 phospho-proteins (Phospho Explorer Antibody Array #KAS02) was performed in hippocampal neurons under two experimental conditions: vehicle and 100 nM...

WebSep 20, 2024 · The anti-ERK1/2 antibody was designed against the epitopes with the same phospho-sites as those detected by the phospho-array. All FFPE samples showed strong immunoreactivity for ERK1/2, with diffuse or regional positivity, and these results were in accordance with the very high relative phosphorylation of ERK2 as detected by the … WebHuman Protein S-Nitrosylation Array G2 The RayBiotech G-Series Human Protein S-Nitrosylation Antibody Array 2 is a multiplex assay on glass slide for the relative detection of cystein s-nitrosylation of 493 different human proteins cell lysate, culture supernatant, serum, plasma and other biological samples.

WebAKT Phospho-Specific Array includes 137 highly specific and well-characterized phosphorylation antibodies in the AKT pathway. Product detail AKT/PKB Signaling Phospho-Specific Array Web2 Citations. Detects 55 phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types but intended for use with cell and tissue lysates. MANUAL / SDS. $901.00. Size. In stock. SKU. AAH-PPP-1-2.

WebProteome Profiler Antibody Arrays are simple and economical multiplex assays for early-stage discovery research. This semiquantitative antibody array allows for the measurement of more than 100 proteins in a single …

WebPhospho Explorer Antibody Array data of KGN cells treated with Hu‐17. (A) Heatmap of proteins differently phosphorylated proteins in KGN cells with Hu‐17 treatment. The data included in the heatmap is beyond cutoff line with the change fold is ≤77% or ≥130%. A is the control group, B is the Hu‐17 treated for 3 h group, C is the Hu ... earl ruthWeb北京智杰方远科技主要为科研领域提供分子生物学、细胞生物学、抗体等相关产品。公司主要代理：Invitrognen、SIGMA、Roche、Abcam、QIAGEN等品牌。我们以“团结诚信,求实创新,质量为本,服务第一”为经营理念。在生命科学领域不断探索，希望不断发展的智杰方远能成为 … css make text moveWebCompared to the conventional methods, our antibody arrays provide a much more cost-effective means for protein research. Investigators can choose from over 40 different arrays to quickly compare protein expression or … earl rutherfordWebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). css make text italicWebApr 13, 2024 · We used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. css make text stay on one lineWebThe antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison. Learn more about cytokine … earl rutlandWebWe used the Phospho Explorer Antibody Array, which encompasses nearly all known signaling pathways, to assess molecular mechanisms underlying LPHN2-induced cardiac differentiation. LPHN2-dependent phosphorylation was the strongest for cyclin-dependent kinase 5 (CDK5) at Tyr15. We identified CDK5, Src, and P38MAPK as key downstream … css make text vertical