Web2 Jun 2008 · SUMO tags are emerging as a viable alternative for increasing both the expression and solubility of otherwise hard-to-express proteins. 9 The SUMO tag can be cleanly excised using SUMO protease, which recognizes the conformation of SUMO protein rather than a specific sequence within SUMO. WebDe-sumoylases remove the SUMO tags, releasing your protein with the desired N-terminal amino acid. Both SUMO tags and de-sumoylases contain 6xHis tags allowing efficient subsequent removal, leaving pure protein. The SUMOPro system is designed for … PROTAC drugs are hetero-bifunctional small molecules that contain two …
SUMO Protease Domain (Ulp1p, M1_K402del) - Kerafast
WebSumo protease cleavage and clean up Change protein buffer to Sumo cleavage buffer. … WebSUMO-tagged protein expression system Fusion Tag: SUMO tag. Purification Method: Ion affinity chromatography. Tag Removal: SUMO protease. Products: Purified recombinant proteins. Quality Characterization: SDS-PAGE. Typical Timeline: 8-12 weeks. Advantages: 1. SUMO fusion tag can be fused to target proteins conveniently and directly. moving background dual monitor overwatch
SUMO protein - Wikipedia
Web1 Nov 2009 · The use of SUMO as a fusion system for protein expression and purification … http://wolfson.huji.ac.il/purification/PDF/Tag_Protein_Purification/SUMO/LifeSensors_SUMOstarInsect.pdf WebThe His14-tag enables Ni2+-chelate affinity chromatography purification of the fusion protein after bacterial expression (described below). The Avi-tag is a 15 amino acid peptide with a single lysine that can be specifically biotinylated using purified E. coli biotin ligase BirA (Beckett et al., 1999; Fairhead and Howarth, 2015). moving background animation css